SCImago Journal & Country Rank

Ranking and Indexing

Rank:
Index Copernicus Value (2010): 5.45
CNCSIS B+ (code 820) since 2007

International Databases:
SCOPUS since 2009
EBSCO Academic Search Complete since 2008
Index Copernicus Journals Master List since 2009
Directory of Open Access Journals (DOAJ) since 2008
ProQuest
Genamics JournalSeek since 18/2008

International Catalogues:
Oxford University's Library since 2003

Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

Who's Online

We have 31 guests online

Index Databases

Assessment of nanoparticles toxicity by flow cytometry using human erythrocytes Print E-mail
Written by Tuşa I., Rugina A., Dobre A.-M., Ciotec A., Calu L., Stan L., Dumitrache F., Fleaca C.,Morjan I, Cotoraci C., Bratosin D.   
Read full article
Article Title: Assessment of nanoparticles toxicity by flow cytometry using human erythrocytes
Authors: Tuşa I., Rugina A., Dobre A.-M.,  Ciotec A., Calu L., Stan L., Dumitrache F., Fleaca C.,Morjan I, Cotoraci C., Bratosin D.
Affiliation: 1National Institute for Biological Science Research and Development, Biotechnological center for cell therapy and regenerative medicine based on stem cell and apoptosis modulators" (BIOREGMED) Bucharest, Romania
2 The National Institute for Laser, Plasma and Radiation Physics, Bucharest, Romania
3”Vasile Goldis” Western University of Arad, Faculty of Medicine, Pharmacy and Dental Medicine, Arad,Romania
4”Vasile Goldis” Western University of Arad, Faculty of Natural Sciences, Arad, Romania
Abstract: The use of nanoparticles in biological and medical applications has increased in the last years. Understanding their potential toxicity and effect on the body is crucial before clinical use. The most used cell culture systems to test their cytotoxicity are of nonhuman origin. For assessing cytotoxicity of nanoparticles we developed a new experimental cell system, the RBCs, and we have evaluated the toxic effects by flow cytometric analysis. Recently, we and other researchers have shown that PCD of human erythrocyte is related to an apoptosis mechanism operating in the absence of mitochondria and lacking the terminal effectors of nuclear apoptosis (erythroptosis also called eryptosis) and to determine human RBC viability, we described a new rapid and sensitive calcein-AM assay by flow cytometric determination. Consequently, in the present study, to evaluate cell-nanoparticles interactions, human RBCs were exposed to different concentrations of nanoparticles and analyzed by flow cytometry, after 3h and 24h incubation endpoints for morphological changes (FSC/SSC) and viability (calcein-AM method). Our results indicate that flow cytometric analysis of RBCs viability using calcein-AM discrimination could provide a rapid and accurate experimental cellular model for effectively screening and evaluating biological responses for in vitro nanotoxicology.
Keywords: human erythrocyte, cell viability, flow cytometric analysis, nanotoxicity, nanoparticles.
References:
*Correspondence: Daniela Bratosin, National Institute for Biological Science Research and Development, Biotechnological center for cell therapy and regenerative medicine based on stem cell and apoptosis modulators" (BIOREGMED) Bucharest, Romania Tel/Fax +40-(021)-2200881, email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it .