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Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

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In vitro conservation of some potato genotypes to improve the ecoeconomic impact of plant biotechnology Print E-mail
Written by Radovet-Salinschi D., Cahita-Cosma D.   
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Article Title: In vitro conservation of some potato genotypes to improve the ecoeconomic impact of plant biotechnology
Authors: Dorina Radoveț-Salinschi, Dorina Cahiță-Cosma
Affiliation: Environmental Protection Agency of Bihor County and postdoctoral researcher at „Vasile Goldiș” Western University of Arad, Romania,
„Vasile Goldiș” Western University of Arad, Romania
Abstract: In plant ”living collection” for micropropagation optimization of potato local variety we used a slow growth technique, for this purpose we used 2 systems: the double layer system (using a basal agarised medium and as supernatant a second layer with silicone oil or paraffin oil, or castor oil), and a normal medium - single layer – by adding in culture media: manithol, sorbithol, AgNO3 or B9. For the double layer system the vitroplantlets were inoculated on simple MS media, without growth regulators, and after 2 weeks – when the plantlet manifests a caulo- and rizogenetic process, we covered the basal agarised medium with a second layer – the oil; for the normal medium – the growth inhibitors were integrated in aseptic MS nutritive medium. This is on going experiment, until this moment the plantlets were vitroconservated 24 weeks in „living collection”; the control lot was subcultived already 2 times: after 12 and 24 weeks of vitroculture. In 24 weeks of vitroplantlets slow growth in „living collection„ did not reach the growth values which control lot achieved in 12 weeks of vitroculture – this showing the efficiency of the presented system.
Keywords: potato, Solanum tuberosum, slow growth, Living Collection, double layer system
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*Correspondence: Radoveț Dorina, Environmental Protection Agency of Bihor County, Protected Areas Department, 25A Dacia Boulevard, Oradea, Romania, Tel. +40-(259)-444590, Fax. +40-(259)-406588, Mobil phone. +40-726 289 789, email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it