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Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

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Localization of CagA effector protein of Helicobacter pylori in infected epithelial cells Print E-mail
Written by Lancrajan I., Kogalniceanu S., Ardelean D., Ardelean G.   
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Article Title: Localization of CagA effector protein of Helicobacter pylori in infected epithelial cells
Authors: Ioana Lancrajan, Svetlana Kogalniceanu, Doru Ardelean, Gavril Ardelean
Affiliation: “Vasile Goldis” Western University Arad, Romania
Abstract: Epidemiological studies have shown the role of CagA-positive Helicobacter pylori (Hp) in development of atrophic gastritis, peptic ulcer disease and gastric carcinoma. CagA effector protein of bacterial pathogen Helicobacter pylori is translocated in host cell via TFSS and subsequently tyrosine phosphorylated. Both phosphorylated and non phosphorylated CagA activate multiple signal transduction pathways promoting disruption of cell-cell contacts, migration and the typical hummingbird phenotype (Bouzrac, Highasi). This study proposed to establish the location of CagA in epithelial cell in culture after infection with Hp. The interaction of extracellular protein CagA from Hp with membrane and cytoskeletal proteins of host cell is to expected, taking in account the multiple contribution of cagA in signal transduction and cell shape modifications, that implied membrane respectively cytoskeletal proteins. The experimental results give one image regarding the traffic of CagA in host cells. According to these data we conclude that Cag A is first, predominantly associated with membranes and cytoskeleton.
Keywords: infection, cell culture, effector, membranes

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*Correspondence: Ioana Lancrajan, Department of Toxicology, “Vasile Goldis” Western University Arad, Faculty of Natural Sciences, 93-95 Rebreanu St., 310396, Arad, Romania, email: Lancrajan_ioana