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Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

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Effect of vitamin E on carbonic anhydrase and superoxide dismutase activity – Experimental research in vitro and in vivo Print E-mail
Written by Mraz C.E., Muresan M., Antonescu A., Micle O., Pallag A., Coltau M., Puscas I.   
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Article Title: Effect of vitamin E on carbonic anhydrase and superoxide dismutase activity – Experimental research in vitro and in vivo
Authors: Camelia Eliza MRAZ1*, Mariana MUREŞAN1, Angela ANTONESCU1, Otilia MICLE1, Annamaria PALLAG2, Marcela COLTĂU3, Ioan PUŞCAŞ3
Affiliation: 1 Department of Preclinical Sciences, Medicine and Pharmacy Faculty, University of Oradea
2 Department of Pharmacy, Medicine and Pharmacy Faculty, University of Oradea
3 City Hospital “Prof.Dr.Ioan Puşcaş” Şimleul Silvaniei, Sălaj
Abstract: The purpose of this study was to determine the effect of vitamin E on erythrocyte carbonic anhydrase (CA) and superoxide dismutase (SOD) activity in vitro and in vivo. In vitro effect was followed by purified isoenzymes CA I and CA II using vitamin solutions with concentrations between 10-8 and 10-4 M and in vivo in mice erythrocytes from Male Sprague-Dawley breed. Groups of rats were given vitamin E (75 mg/kg/day, i.g.), 3,4-Benzpyren (200 ppm/day, i.g.), Vitamin E + 3,4-Benzpyren (75 mg/kg/day + 200 ppm/day, i.g), and a Control group only Placebo. Measurement of enzymes activity was done by following the hydration reaction of CO2 (stopped-flow method) for CA, respectively the enzymatic inhibition of oxidation of epinephrine to adrenochrome for SOD. In vitro there is a direct activation of CA by dose-response relationship maximum at concentration 10-4 M. Both in vitro and in vivo, vitamin E produced a increase in CA I and CA II activity, and the effect is stronger on CA II isoenzyme. In vivo studies show that vitamin E decreases CA and SOD inhibition caused by Benzpyren.
Keywords: vitamin, carbonic anhydrase, superoxide dismutase, activity
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*Correspondence: Mraz Camelia, University of Oradea, Medicine and Pharmacy Faculty, Department of Preclinical Sciences, 1 December St. no. 10, 410068, Oradea, Romania, Tel. +40-(259)- 415 680/157, email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it