SCImago Journal & Country Rank

Ranking and Indexing

Rank:
Index Copernicus Value (2010): 5.45
CNCSIS B+ (code 820) since 2007

International Databases:
SCOPUS since 2009
EBSCO Academic Search Complete since 2008
Index Copernicus Journals Master List since 2009
Directory of Open Access Journals (DOAJ) since 2008
ProQuest
Genamics JournalSeek since 18/2008

International Catalogues:
Oxford University's Library since 2003

Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

Who's Online

We have 299 guests online

Index Databases

Effect of vitamin E on carbonic anhydrase and superoxide dismutase activity – Experimental research in vitro and in vivo Print E-mail
Written by Mraz C.E., Muresan M., Antonescu A., Micle O., Pallag A., Coltau M., Puscas I.   
Read full article
Article Title: Effect of vitamin E on carbonic anhydrase and superoxide dismutase activity – Experimental research in vitro and in vivo
Authors: Camelia Eliza MRAZ1*, Mariana MUREŞAN1, Angela ANTONESCU1, Otilia MICLE1, Annamaria PALLAG2, Marcela COLTĂU3, Ioan PUŞCAŞ3
Affiliation: 1 Department of Preclinical Sciences, Medicine and Pharmacy Faculty, University of Oradea
2 Department of Pharmacy, Medicine and Pharmacy Faculty, University of Oradea
3 City Hospital “Prof.Dr.Ioan Puşcaş” Şimleul Silvaniei, Sălaj
Abstract: The purpose of this study was to determine the effect of vitamin E on erythrocyte carbonic anhydrase (CA) and superoxide dismutase (SOD) activity in vitro and in vivo. In vitro effect was followed by purified isoenzymes CA I and CA II using vitamin solutions with concentrations between 10-8 and 10-4 M and in vivo in mice erythrocytes from Male Sprague-Dawley breed. Groups of rats were given vitamin E (75 mg/kg/day, i.g.), 3,4-Benzpyren (200 ppm/day, i.g.), Vitamin E + 3,4-Benzpyren (75 mg/kg/day + 200 ppm/day, i.g), and a Control group only Placebo. Measurement of enzymes activity was done by following the hydration reaction of CO2 (stopped-flow method) for CA, respectively the enzymatic inhibition of oxidation of epinephrine to adrenochrome for SOD. In vitro there is a direct activation of CA by dose-response relationship maximum at concentration 10-4 M. Both in vitro and in vivo, vitamin E produced a increase in CA I and CA II activity, and the effect is stronger on CA II isoenzyme. In vivo studies show that vitamin E decreases CA and SOD inhibition caused by Benzpyren.
Keywords: vitamin, carbonic anhydrase, superoxide dismutase, activity
References: Breton S., The Cellular Physiology of Carbonic Anhydrases, JOP J Pancreas (Online), 2(4 Suppl):159-164, 2001
Brigelius-Flohe R., Kelly F.J., Salonen J., Neuzil J., Zingg J.M., Azzi A., The European perspective on vitamin E: current knowledge and future research. American Journal of Clinical Nutrition, 76, pp.703–716, 2002
Ciftci M., Bülbül M., Gül M., Gümüstekin K., Dane S., Süleyman H., Effects of nicotine and vitamin E on carbonic anhydrase activity in some rat tissues in vivo and in vitro, J Enzyme Inhib Med Chem, 2005, 20, pp.103–108
Gilmour K.M. Perspectives on carbonic anhydrase, Comp. Biochem Physiol a Mol Integr Physiology, 157, 3, 193-197, 2010
Johnson F., Giulivi C., Superoxide dismutases and their impact upon human health, Mol Asp Med, 26, pp.340–352, 2005
Kadkhodaee M., Khastar H., Arab H.A., Ghaznavi R., Zahmatkesh M., Mahdavi-Mazdeh M., Antioxidant vitamins preserve superoxide dismutase activities in gentamicin induced nephrotoxicity, Transplant Proc, 39, pp.864–865, 2007
Kashani Z. H., Imanpoor M. R., Shabani A., Gorgin S., Effect of dietary vitamin C, E and highly unsaturated fatty acid on growth and survival of goldfish (Carassius auratus), AACL Bioflux, 3(4), pp.281-288, 2010
Kataria N., Kataria A. K., Pandey N., Gupta P., Serum biomarkers of physiological defense against reactive oxygen species during environmental stress in Indian dromedaries, HVM Bioflux, 2(2), pp.55-60, 2010
Khalifah R.G., The carbon dioxide hydration activity of carbonic anhydrase, J Biol Chem, 246, pp.2561-2573, 1971
Kim H.S., Kwack S.J., Lee B.M., Lipid peroxidation, antioxidant enzymes, and benzo[a]pyrene-quinones in the blood of rats treated with benzo[a]pyrene, Chem Biol Interact, 127, pp.139–150, 2000
Landis G.N., Tower J., Superoxide dismutase evolution and life span regulation, Mech Ageing Dev, 126, pp.365–379, 2005
Lonn E., Bosch J., Yusuf S., Effects of long-term vitamin E supplementation on cardiovascular events and cancer: a randomized controlled trial, Journal of the American Medical Association, 293, pp.1338–1347, 2005
McCord J.M., Fridovich I., Superoxide dismutase - An enzymatic function for erythrocuprein (hemocupreine), J Biol Chem, 244, pp.6049-6055, 1969
Mehrad B., Sudagar M., Dietary vitamin E requirement, fish performance and reproduction of guppy (Poecilia reticulata), AACL Bioflux, 3(3), pp.239-246, 2010
Miller K.P., Ramos K.S., Impact of cellular metabolism on the biological effects of benzo[a]pyrene and related hydrocarbons, Drug Metab Rev, 33, pp.1-35, 2001
Misra H.P., Fridovich I., The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase, J Biol Chem, 247, pp.3170-3175, 1972
Parkkila S., Significance of pH regulation and carbonic anhydrases in tumour progression and implications for diagnostic and therapeutic approaches, BJU International, 101, 4, pp.16-21, 2008
Puscas L., Coltau M., Domuta G., Rapid method for differentiation of carbonic anhydrase I from carbonic anhydrase II activity, Anal Letter, 32(5), 915-923, 1999.
Puşcaş I., Coltău M., Maghiar A., Cladovan C., Inhibition and activation of superoxide dismutase and of carbonic anhydrase in ex vivo with benzo(a)pyrene and cyclophosphamide in pacients with gastric cancer as compared to controls, Romanian J Gastroenterology, 21, 1995
Skrzycki M., Majewska M., Podsiad M., Czeczot H., Expression and activity of superoxide dismutase isoenzymes in colorectal cancer, Acta Biochim Pol, 56, pp.663–670, 2009
Supuran C.T., Carbonic anhydrases novel therapeutic applications for inhibitors and activators, Nature Reviews Drug Discovery, 7, 168-181, 2008.
Zelko I.N., Mariani T.J., Folz R.J., Superoxide dismutase multigene family: A comparison of the Cu,ZnSOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression, Free Radic Biol Med, 33, pp. 337–349, 2002.
*Correspondence: Mraz Camelia, University of Oradea, Medicine and Pharmacy Faculty, Department of Preclinical Sciences, 1 December St. no. 10, 410068, Oradea, Romania, Tel. +40-(259)- 415 680/157, email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it