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Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

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Initiation of Begonia semperflorens cv. ‚Ambassador’ white vitroculture from plant apex of zygote origin Print E-mail
Written by Gergely J.E., Cachita-Cosma D.   
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Article Title: Initiation of Begonia semperflorens cv. ‚Ambassador’ white vitroculture from plant apex of zygote origin
Authors: Julieta - Emilia Gergely1*, Dorina Cachiţă-Cosma2
Affiliation: 1 University of Oradea, Faculty of Science, Department of Biology. Oradea. Romania
2 “Vasile Goldiş” Western University of Arad. Arad. Romania
Abstract: The initiation of Begonia semperflorens cv. ‘Ambassador’ White vitroculture was made by in vitro cultivation of some explants consisting in apical minicuttings, approx. 3-4 mm size, taken from 30 days old seedlings, derived from germinated seeds in aseptic Petri capsules, on filter paper moistened with sterile water. Explants were inoculated and grown – for 30 days – on Murashige-Skoog mineral medium (1962), modified, with added vitamins, sucrose, agar-agar, with or without growth regulators, namely the addition of indolebutyric acid (IBA) and/ or with thidiazuron (TDZ) 0.5 mg/l each. The minicuttings consisted of upper side of hypocotile, which held the bud strain of plant, the two epigeal cotyledons being inserted into its basal zone. After 30 days in vitro culture, the best results in terms of phylogenesis were obtained from the mineral basic medium culture with the addition of 0.5 mg / l TDZ, the number of regenerated leaves was much higher than that recorded on the other experimental variants. But the most balanced regeneration on buds, leaves respectively, as well as on roots was obtained from mineral basal medium culture supplemented with 0.5 mg / l TDZ in combination with 0.5 mg / AIB. If the culture was present 2.5mg /l.4-dichlorophenoxyacetic acid (2,4-D), the morphogenesis consisted only in calusogenesis.
Keywords: Begonia semperflorens cv. ’Ambassador’ White, cytokine, auxine, micropropagation
References: Appelgren M, (1984) Tissue culture of ornamental plants with special reference to flowering potted plants. In: Micropropagation of Selected Root Crops, Palms, Citrus and Ornamental Species, Food and Agriculture Organization of the United Nations, pp.177-191.
Arora YK, Nakao S, Nakajima T, (1970) Perpetuation of Begonia rex by aseptic culture with micro-leaf cuttings under various conditions of auxin and cytokinin. Japan. J. Breed., 20, pp.275-281.
Berghoef J, Bruinsma J, (1980) Nutritional rather than hormonal regulation of sexual expression in Begonia franconis. Phytomorphology, 30, pp.231-236.
Bigot C, (1981) Multiplication végétative in vitro de Begonia x hiemalis (Rieger et Schwabenland). II. Conformité des plantlets élevées en serre. Agronomie, 1, pp.441-447.
Cachiţă CD, Deliu C, Tican RL, Ardelean A, (2004) Tratat de biotehnologie vegetală, vol.I, Editura Dacia, Cluj-Napoca, pp.29-154.
Heide OM, (1967) The auxin level of Begonia leaves in relation to their relation to their regeneration ability. Physiol. Plant., 20, pp.886-902.
Loo S, (1982) Perspective on the application of plant cell land tissue culture. In: Plant Tissue Culture, 1982, (Fujiwara A. eds.), Maruzen Publishing Co., Tokyo, pp. 19-24.
Mikkelsen EP, Sink KC, (1978) In vitro propagation of Rieger Elatior begonias. Hortic. Sci., 13 (3), pp.242-244.
Murashige T, (1974) Plant propagation through tissue cultures. Ann. Rev. Plant Physiol., 25, pp. 135-166.
Murashige T, Skoog F, (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15(3), pp. 473-497.
Peak DE, Cumming BG, (1984) In vitro propagation of Begonia×tuberhybrida from leaf sections. Hortic. Sci., 19, pp 395–397.
Pierik RLM, Tettero FAA, (1987) Vegetative propagation of Begonia venosa Skan in vitro from inflorescence explants. Plant Cell Tis. Org. Cult.; 10, pp.135-142.
Reuter G, Bhandari NN, (1981) Organogenesis and histogenesis of adventitious organs induced on leaf blade segments of Begonia elatior hybrids (Begonia×hiemalis) in tissue culture, Gartenbauwissenschaft, 46, pp.241–249.
Ringe F, (1972) A further contribution to the question of cytochinin-like activity of 8-quinolinol sulphate. Experientia, 28, pp.234-235.
Ringe F, Nitsch JP, (1968) Conditions leading to flower formation on excised Begonia fragments cultured in vitro. Plant Cell Physiol., 9, pp.45-57.
Roest S, (1977) Vegetative propagation in vitro and its significance for mutation breeding. Acta Hortic., 78, pp.349-359.
Romocea JE, Pop L, Gergely I, (2010) Initiation of Begonia erythrophylla L. vitroculture from axillary buds, Analele Universităţii din Oradea, Fascicola Biologie, Tom.; 17, (2), pp.324-328.
Rout GR, Jain SM, (2004) Micropropagation of ornamental plants cut flowers. Propag. Ornam. Plant., 4(2), pp.3-28.
Simmonds J, Werry T, (1987) Liquid shake cultures for improved micropropagation of Begonia×hiemalis. Hortic. Sci., 22, pp.122-124.
Takayama S, Misawa M, (1981) Mass propagation of Begonia×hiemalis plantlets by shake culture. Plant Cell Physiol., (22), pp.461-467.
Takayama S, Misawa M, (1982) Factors affecting differentiation in vitro and a mass-propagation scheme for Begonia×hiemalis. Sci. Hortic., (16), pp.65-75.
Welander T, (1977) In vitro organogenesis in explants from different cultivars of Begonia×hiemalis. Physiol. Plant., 41, pp.142-145.
Welander T, (1981) Effect of polarity on and origin of in vitro formed organs in ex plants of Begonia elatior hybr. Swed. J. Agric. Res., 11, pp. 77-83.
*Correspondence: Julieta - Emilia Gergely, University of Oradea, Faculty of Science, Department of Biology, 1 Universităţii Str., zip code: 410087, Oradea, Romania, phone: 0040259408448, e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it