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Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

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Studies regarding the influence of different wavelengths of LEDs light on regenerative and... Print E-mail
Written by Vidican T.I., Cachita-Cosma D.   
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Article Title: Studies regarding the influence of different wavelengths of LEDs light on regenerative and morphogenetic processes in in vitro cultures of Echinopsis chamaecereus F. Lutea
Authors: Teodora Iuliana Vidican1, Dorina Cachita-Cosma2
Affiliation: 1 University of Oradea, Romania
2 “Vasile Goldis” Western University of Arad, Romania
Abstract: Echinopsis chamaecereus f. lutea is a yellow, ornamental cactus species, which belongs to the group of mutant, chlorophyll-deficient cacti. Their inability to synthesize chlorophyll makes these chlorophyll-deficient cacti survive only if they are grafted onto adequate stock which contains chlorophyll. Chlorophyll-deficient cacti are multiplied through “in vitro” cloning. With regard to the establishment of an “in vitro” culture of Echinopsis chamaecereus f. lutea, from the parent plant grown in the greenhouse, we sampled 1-cm explants, which were used as stem segments that were inoculated on an aseptic agarose medium with macroelements and Murashige-Skoog FeEDTA (1962), Heller microelements (1953), vitamins – pyridoxine HCl, thyamine HCl, and nicotinic acid (1 mg/l of each) – m-inositol and sucrose, without growth regulators. In the grow room, the tissue cultures were illuminated with light-emitting diodes (LEDs) of different colors (yellow, red, green, blue or white), with a light intensity of 1000 lx. The evolution (rhizogenesis, callogenesis, and caulogenesis) of the in vitro cultures was monitored for 90 days, tracking the differences in reactivity to the different wavelengths of LEDs illumination. Cultures exposed to white light emitted by fluorescent tubes served as the control sample. After 90 days, compared to the differentiation of the control sample exposed to white light from fluorescent tubes, the samples grown in the presence of green or blue LEDs demonstrated statistically significant increases in the growth of the stems; under red or green LEDs illumination, calusogenesis intensified, while under white or yellow LEDs illumination, the rate of development of the stems was not significantly different from the growth of the control samples. Rhizogenesis was not observed in any of the samples.
Keywords: cacti, in vitro culture, LEDs light source
References: Cachiţă C.D., Deliu C., Rakosy-Tican L., Ardelean A., 2004, Tratat de bioteh-nologie vegetală, vol. I, Ed. Dacia, Cluj-Napoca, pp. 29-196.
Catrina I., Popa A., 1987, Concepţii şi metode noi în silvicultură privind conversia energiei solare şi creşterea randamentului fotosintezei, Min. Silv. ICAS Bucuresti, Ed. Ceres, Bucuresti, pp. 36.
Copăcescu V.S., 2001, Cactuşii, monografie; Ed. Ceres, Bucuresti, p. 11-517.
Heller R., 1953, Rescherches sur la nutrition minérale des tissus végétaux cultives in vitro. Ann.Sci. Nat. Bot. Veg. Ser., vol. II, pp. 1-5.
Kornilova L.P., 2008, Grafting on Pereskiopsis, Cultivar, vol.4, nr. 41, publicat online: http://www.lapshin.org/cultivar/N41/index-e.htm.
Jagers F., 2007, Influence the color of light emitted by colored LEDs on the development of in vitro plant, Fruit & Veg Tech 7.5, vol 8, Olanda, pp. 2-8.
Michalczuk, B., Michalczuk, L., 2000, The effect of light quality on regeneration rate and plantled development in transgenic Petunia ‘Revolution’ (Surfina type), Acta Hort. 530, Cork, Ireland, pp.397-402.
Miler N., Zalewska M., 2006, The influence of light colour on micropro-pagation of chrysanthemum, Acta Hort. 725, Debrecen, Hungary, pp. 347-350.
Murashige T., Skoog F., 1962 – A revised medium for rapid growth and biossays with tabacco tissue cultures, Physiol. Plant., vol. 15, pp. 473 – 497.
Pop L., Cachiţă D., 2007, Preliminary research concerning the reaction of Sequoia sempervirens vitrocultivatures to „high brightness” LEDs illumina-tion, Analele Universităţii din Craiova, pp. 215-219.
Shemorakov N., 2003, Cultivar’s classification by stem color, Cultivar, vol. 2, nr. 18, Published Online: http://www.lapshin.org/cultivar/N18/class-e.
Tarhon A., 1987, Elemente teoretice şi aplicaţii numerice în chimia fizică, Ed. Tehnică, Bucureşti.
Winslow R.B., 2002, Plant photoreceptors: proteins that perceive information vital for plant development from the light environment, The Urban Wildrands group and Ecological Consequences of Artificial Night Lighting, Department of Plant Biology, Carnegie Institution of Washington, USA, pp. 260.
*Correspondence: Teodora Iuliana Vidican, University of Oradea, C.P. nr. 114, Oficiul Postal 1, str. Universitatii nr. 1, e-mail : This e-mail address is being protected from spambots. You need JavaScript enabled to view it