SCImago Journal & Country Rank

Ranking and Indexing

Index Copernicus Value (2010): 5.45
CNCSIS B+ (code 820) since 2007

International Databases:
SCOPUS since 2009
EBSCO Academic Search Complete since 2008
Index Copernicus Journals Master List since 2009
Directory of Open Access Journals (DOAJ) since 2008
Genamics JournalSeek since 18/2008

International Catalogues:
Oxford University's Library since 2003

Journal Info

Title: Studia Universitatis Vasile Goldis, Seria Stiintele Vietii (Life Sciences Series)
Abbreviated title: Studia Univ. VG, SSV
Publisher: "Vasile Goldis" University Press
Owner: Western University "Vasile Goldis" Arad, Romania
ISSN: 1584-2363
e-ISSN: 1842-7863
ISSN-L: 1584-2363

Who's Online

We have 91 guests online

Index Databases

In vitro effects of nitric oxide synthase inhibition by L-NAME on human internal mammary arteries rings Print E-mail

Authors: Germaine SAVOIU1, Constantin CRĂCIUN2, Carmen CRISTESCU3, Corina ȘERBAN1*, Daniela IONESCU4, Ovidiu FIRA-MLADINESCU1, Lavinia NOVEANU5, Danina MUNTEAN1, Georgeta MIHALAȘ5

Affiliation: 1 Department of Pathophysiology, University of Medicine and Pharmacy “Victor Babes” Timisoara, Romania; 2 Electron Microscopy Center, “Babes-Bolyai“ University, Cluj-Napoca, Romania; 3 Department of Clinical Pharmacy, University of Medicine and Pharmacy “Victor Babes” Timisoara, Romania; 4 Department of Toxicology, University of Medicine and Pharmacy “Victor Babes” Timisoara, Romania; 5 Department of Physiology, University of Medicine and Pharmacy “Victor Babes” Timisoara


Read full article


ABSTRACT. Human internal mammary arteries (IMA) are relatively protected from atherosclerosis. The aim of the present study was to investigate the effect and mechanism of action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on human mammary artery (IMA) segments. Segments of IMA were obtained from 12 patients who underwent coronary artery bypass graft surgery. They were cut into rings, suspended between two wire hooks in organ bath chambers and constricted submaximally with Phenylephrine. Isometric tension was continuously measured with an isometric force transducer connected to a computer-based data acquisition system. The dose-effect graphs were determined for the following substances: 5¢ADP ± L-NAME in concentrations between 10-9 and 10-4M on the precontracted rings by 10-5M Phenylephrine and NSP ± L-NAME for concentrations between 10-9 and 10-4M, on the precontracted rings by 10-5M Phenylephrine. The administration of the pharmacological agents was performed in a cumulative manner, the concentration being expressed in absolute values (cN), and the relaxation as % of the tension induced by the pre-contraction with 10-5M Phenylephrine. In our study, the incubation of the human artery mammary rings for 30 minutes with 10-5M L-NAME determined a significant increase of contractile response to 10-5M Phenylephrine (p < 0.001), a significant decrease of response to 5’ADP (p < 0.001) and absence of modification of the endothelial-independent response to NSP (p = 0.03). The results of our study showed that, in the case of a rigorous standardized methodology, the experimental protocol study of a vasomotor function of endothelium, in our case human arterial mammary artery, it can reveal endothelial dysfunction. The exposure to an inhibitor of NO Synthase Inhibitor L-NAME, (N (G)-nitro-L-arginine methyl ester), has to attenuate significant the vasodilator response of the vascular ring to Phenylephrine.


Keywords: endothelium, nitric oxide, L-NAME